2021-2-8 · The purified PCR products were fused to the linearized pRGEB32- GhU6.9 vector using ClonExpress II One Step Cloning Kit. Each assembly group should contain at least 200 monocolonies of Bacterial-host-competent cells grown in a selection medium. All resistant cells were then harvested in 100 mL lysogeny broth (LB) medium and cultured over night
The purified PCR fragments were ligated with the linearized plasmid pMA5-sat via Gibson assembly using the ClonExpress II One-Step Cloning Kit and the resultant plasmid was used to transform competent E. coli JM109 cells. The recombinant plasmids
ClonExpress II One Step Cloning Kit C112-02 -Equl_AdvancedBioMatrix_DivBio_Drummond_Genie_Glascol_Megazyme_Phadebas_Worthington Megazyme AUVON ElisaSystems Phadebas Phadebas EBPI TDzyme
2017-4-13 · The SPpr1-eGFP-T35S fragment was subsequently cloned into the SpeI/XbaI sites of Sol2095 with the ClonExpress II One-Step Cloning Kit yielding the plasmid pLOCGex (Fig. S2A). Another binary vector pLOCG without plant PR1 signal peptide was generated by amplification of the eGFP-T35S cassette with the primers GFP_pBin_F and T35S_pBin_R using
ClonExpress II One Step Cloning (Vazyme #C112) is a simple fast and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly the vector is linearized at the cloning
2021-6-18 · ClonExpress ® II Exnase ® II PCR5 - PCR5 3 15bp 20bp
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
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2020-2-3 · PCR products were inserted into lined pART27 GFP using ClonExpress II One Step Cloning Kit (Vazyme China). These positive clones were transformed into Agrobacterium tumefaciens strain GV3101. The pART27 GFP transformed Agrobacterium
ClonExpress II One Step Cloning Kit. 73.00 €122.00 €. excl. VAT plus shipping. Size. Choose an option 25 RXNS 50 RXNS. Clear. ClonExpress II One Step Cloning Kit quantity. Add to cart. Categories Cloning Mutagenesis Fast Cloning Molecular
C112-01 ClonExpress® II One Step Cloning Kit C112-01 ClonExpress® II One Step Cloning Kit 1
As a new generation recombinant cloning kit ClonExpress® II has a unique non-ligase dependent system which greatly reduces the background of vector self-ligation and the positive rate can reach more than 95 . The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of
As a new generation recombinant cloning kit ClonExpress® II has a unique non-ligase dependent system which greatly reduces the background of vector self-ligation and the positive rate can reach more than 95 . The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of
2021-3-15 · C112 ClonExpress II One Step Cloning kit.pdf Created Date 6/5/2019 1 12 08 PM
2021-7-9 · ClonExpress II One Step Cloning Kit 25 rxn. Home » Shop » ClonExpress II One Step Cloning Kit 25 rxn. ClonExpress® technology is a simple fast and efficient DNA seamless cloning technology which can clone the insert into any position of any vector. Linearize the vector and place the insert 5 in the forward/reverse PCR primer Introduce
2021-7-22 · ClonExpress II One Step Cloning Kit C112 Vazyme 6001000 ClonExpress MultiS One Step Cloning Kit
Clonexpress Ii One Step Cloning Ki Clonexpress Ii One Step Cloning Kit Clonexpress Ii One Step Cloning Kit Cells Direct One Step Qrt Pcr Kit Accurate Chemicals One Step Cell Direct One Stepo Qrt Pcr Igg Igm Oneblood Antibodies Test Anti Bodies Buy Antibodies Virus Antibodies Only 23 Months Anti Human Only Antibodies Raised In Goats
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2016-8-5 · ClonExpress II One Step Cloning Kit (Vazyme Biotech Co. Ltd). Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50 ng linear plasmid 100 ng PCR fragment and ddH2O was incubated at 37oC for 30 min and the resulting solution was used to transform chemically competent E. coli DH5α cells. Colony PCR and
2021-3-15 · C112 ClonExpress II One Step Cloning kit.pdf Created Date 6/5/2019 1 12 08 PM
C112-01 ClonExpress® II One Step Cloning Kit C112-01 ClonExpress® II One Step Cloning Kit 1
ClonExpress-II One Step Cloning Kit (Vazyme Nanjing China) respectively. For fatty acids analysis in yeast HMS1 and HMS1I were cloned into pYES2 and pRS416 vectors respec-tively. All primers are shown in Supporting Information Table S1. Expression analyses Total RNA was isolated from rice anthers or spikelets using Tri-zol reagent (Invitrogen).
2017-5-12 · The assembled fragment was inserted to the linearized pRGEB32 using ClonExpress® II One Step Cloning Kit (Vazyme Nanjing China) thus generating pRGEB32-GhU6.9 vector. It has a hpt selection marker and was used to target a reporter gene DsRed2 which was firstly transformed into cotton with a vector carrying NPT II (Neomycin
2021-7-9 · ClonExpress II One Step Cloning Kit 25 rxn. Home » Shop » ClonExpress II One Step Cloning Kit 25 rxn. ClonExpress® technology is a simple fast and efficient DNA seamless cloning technology which can clone the insert into any position of any vector. Linearize the vector and place the insert 5 in the forward/reverse PCR primer Introduce
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2021-7-20 · ClonExpress ® DNA ClonExpress ® II Exnase ® II
The fragment was ligated into the HindIII-digested mini-CTX-lacZ plasmid following the instruction of the ClonExpress II One Step Cloning Kit (Vazyme China) generating mini-CTX-Ptat-lacZ. The I-F cas operon including its native promoter (8.693-kb) was obtained by PCR employing PA154197 genomic DNA as the template.
2021-3-16 · obtained PCR products are compatible with ClonExpress II One Step Cloning Kit series (Vazyme #C112 #C113). The PCR products contain A at the 3 -end and can be directly cloned into T-Vectors. One unit (U) is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble products in 30 min at 74 ℃with
Clonexpress Ii One Step Cloning Ki Clonexpress Ii One Step Cloning Kit Clonexpress Ii One Step Cloning Kit Cells Direct One Step Qrt Pcr Kit Accurate Chemicals One Step Cell Direct One Stepo Qrt Pcr Igg Igm Oneblood Antibodies Test Anti Bodies Buy Antibodies Virus Antibodies Only 23 Months Anti Human Only Antibodies Raised In Goats
As a new generation recombinant cloning kit ClonExpress® II has a unique non-ligase dependent system which greatly reduces the background of vector
ClonExpress® II One Step Cloning Kit. . 95 . 50 bp-10 kbp DNA. PCR. .
Clonexpress Ii One Step Cloning Ki Clonexpress Ii One Step Cloning Kit Clonexpress Ii One Step Cloning Kit Cells Direct One Step Qrt Pcr Kit Accurate Chemicals One Step Cell Direct One Stepo Qrt Pcr Igg Igm Oneblood Antibodies Test Anti Bodies Buy Antibodies Virus Antibodies Only 23 Months Anti Human Only Antibodies Raised In Goats
2021-5-28 · Adopting C112 ClonExpress II One Step Cloning Kit (Vazyme Biotech China) homologous recombination enzyme truncated sequence and the linear carrier s connection and the fusion plasmids were sequenced by Sangon Biotech Company (Sangon Biotech China).
2021-7-22 · ClonExpress II One Step Cloning Kit C112 Vazyme 6001000 ClonExpress MultiS One Step Cloning Kit
Clonexpress Ii One Step Cloning Ki Clonexpress Ii One Step Cloning Kit Clonexpress Ii One Step Cloning Kit Cells Direct One Step Qrt Pcr Kit Accurate Chemicals One Step Cell Direct One Stepo Qrt Pcr Igg Igm Oneblood Antibodies Test Anti Bodies Buy Antibodies Virus Antibodies Only 23 Months Anti Human Only Antibodies Raised In Goats
ClonExpress® II One Step Cloning Kit. . 95 . 50 bp-10 kbp DNA. PCR. .
ClonExpress II One Step Cloning Kit 618.00 Phanta Max Super-Fidelity DNA Polymerase 618.00 615.00 2 Phanta Max Master Mix 615.00 700.00 Ultra GelRed (10000 ) 700.00 60.00 DL2000 Plus DNA Marker 60.00 60.00 DL5000 DNA Marker 60.00 75.00
ClonExpress II One Step Cloning Kit and Phanta Max (p515) DNA polymerases were purchased from Vazyme Biotech Co. Ltd. (Nanjing China). Oligonucleotides were synthesized by GENEWIZ Bio Inc. (Suzhou China). Strains plasmids and media. The strains and plasmids involved in this study are listed in table S3. The MP6 and CRISPR systems for gene
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