Alexa Fluor® 488 is a bright green-fluorescent dye with excitation ideally suited for the 488 nm laser line. Used for stable signal generation in imaging and flow cytometry Alexa Fluor® 488 dye is water soluble and pH-insensitive from pH 4 to pH 10.
Alexa Fluor® 488conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter and more photostable than FITC Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green
2021-7-18 · Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g. a 530/30-nm bandpass
2021-7-23 · Alexa Fluor® 488 CD4 EPR6855 (Alexa Fluor® 488 Anti-CD4 antibody EPR6855 ) "" 2016 IVD POCT
Alexa Fluor ® 488 conjugated antibodies advantages compared to Fluorescein isothiocyanate (FITC) make it the most used green fluorophore for fluorescent cell staining. Comparable spectral properties and quantum yield. Brighter and more photostable when conjugated to antibodies.
Before use Alexa 488 is dissolved at 50 m M in DMSO. 7. Alexa 488 solution is added to 2 mg/mL of F-actin at a final concentration of 240 μ M. It is recommended that DMSO be kept below 0.5 in the actin solution. 8. The mixture is stirred gently using a magnetic stirrer for 15 h at 4 °C in the dark.
2021-7-19 · Ab150129 Alexa Fluor® 488 donkey anti-goat IgG (H L) (shown in green) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
2006-12-1 · Alexa Fluor® 488 Protein Labeling Kit 4 3.3 Calculate the degree of labeling moles dye per mole protein = A 494 dilution factor where 71 000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. For IgGs we find that labeling with 4–9 moles of Alexa Fluor® 488
Anti-rabbit IgG (H L) F (ab )2 Fragment (Alexa Fluor ® 488 Conjugate) was used as a secondary antibody. High content analysis of HeLa cells exposed to varying concentrations of staurosporine for 3hr. With increasing concentrations of staurosporine a significant decrease ( 2.5 fold) in phospho-MAPKAPK-2 signal as compared to the untreated
Preparation. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. Concentration. 0.5 mg/ml. Storage Handling. The antibody solution should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light.
2021-7-18 · Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g. a 530/30-nm bandpass
488. SNAP-Surface ® Alexa Fluor ® 488 is a photostable green fluorescent substrate that can be used to label SNAP-tag ® fusion proteins in solution or on the surface of living cells. This is a cell impermeable substrate based on BG-Alexa Fluor 488. It is suitable for standard fluorescein filter sets. It has an excitation maximum at 496 nm
2021-7-23 · Alexa Fluor® 488 CD4 EPR6855 (Alexa Fluor® 488 Anti-CD4 antibody EPR6855 ) "" 2016 IVD POCT
Alexa Fluor® 488. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC Cy2 and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing
2006-12-1 · Alexa Fluor® 488 Protein Labeling Kit 4 3.3 Calculate the degree of labeling moles dye per mole protein = A 494 dilution factor where 71 000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. For IgGs we find that labeling with 4–9 moles of Alexa Fluor® 488
This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 488 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the
Alexa-488 C30H27N4O12S2 CID 102384756structure chemical names physical and chemical properties classification patents literature biological activities
2021-7-18 · Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g. a 530/30-nm bandpass
The secondary antibody (green) was ab150077 Alexa Fluor ® 488 goat anti-rabbit IgG (H L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of
2018-12-13 · Alexa Fluor® FluoroNanogold™ is a unique dual-purpose probe. Alexa Fluor® 488 FluoroNanogold™ reagents consist of affinity-purified Fab fragments conjugated to both Alexa Fluor® 488 dye (green) and the 1.4 nm Nanogold® particle.1 An Alexa Fluor® 488 FluoroNanogold™ Fab conjugate is shown in Figure 1.
Alexa Fluor 488 spectrumAlexa Fluor 488 is a fluo excitation and emission wavelengths using the interactive Spectrum ViewerA web application for viewing and comparing spectra of various fluorescent compounds. Exportable as image or link.
Invitrogen Alexa Fluor 488 dye is a bright green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry Alexa Fluor 488 dye is pH-insensitive over a wide molar range.
Alexa Fluor® 488. Amax 493 Emax 519nm. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC Cy2 and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures
2005-10-22 · tion of Alexa Fluor 488 dye assayed in 100 mM sodium phos-phate pH 8.0. Maximal quenching corresponds to more than 90 of the fluorescence of the free dye. Due to steric hindrance or limited accessibility of the Alexa Fluor 488 dye maximal fluorescence quenching of Alexa Fluor 488 conjugates may be significantly less. Storage and Handling
Alexa Fluor 488 spectrumAlexa Fluor 488 is a fluo excitation and emission wavelengths using the interactive Spectrum ViewerA web application for viewing and comparing spectra of various fluorescent compounds. Exportable as image or link.
Confocal immunofluorescent analysis of mouse hippocampus using F4/80 (BM8.1) Rat mAb #71299 detected with Anti-rat IgG (H L) (Alexa Fluor ® 488 Conjugate) #4416 (green) and Myelin Basic Protein (D8X4Q) XP ® Rabbit mAb #78896 detected with Anti-rabbit IgG (H L) F(ab ) 2 Fragment (Alexa Fluor ® 555 Conjugate) #4413 (red). Sections were mounted in ProLong ® Gold Antifade Reagent with DAPI
2020-8-12 · Alexa Fluor® 488 annexin V/Dead Cell Apoptosis Kit with Alexa® Fluor 488 annexin V and PI for Flow Cytometry Catalog nos. V13241 and V13245 Introduction Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apoptosis is implicated in disease states such as
2021-7-19 · Ab150129 Alexa Fluor® 488 donkey anti-goat IgG (H L) (shown in green) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® 488. Amax 493 Emax 519nm. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC Cy2 and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures
Anti-rabbit IgG (H L) F (ab )2 Fragment (Alexa Fluor ® 488 Conjugate) was used as a secondary antibody. High content analysis of HeLa cells exposed to varying concentrations of staurosporine for 3hr. With increasing concentrations of staurosporine a significant decrease ( 2.5 fold) in phospho-MAPKAPK-2 signal as compared to the untreated
Preparation. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. Concentration. 0.5 mg/ml. Storage Handling. The antibody solution should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light.
Alexa Fluor ® 488 Phalloidin allows researchers to fluorescently stain the cytoskeleton through the binding of phalloidin to F-actin. This product is intended for use on fixed and permeabilized samples due to the toxicity associated with phalloidin. After reconstitution the stock solution provides enough material to perform 300 assays based on
Alexa-488 C30H27N4O12S2 CID 102384756structure chemical names physical and chemical properties classification patents literature biological activities
Alexa Fluor® 488conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter and more photostable than FITC Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green
15 hours ago · Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g. a 530/30-nm bandpass filter). Please ensure that your instrument s configurations (lasers and optical filters) are appropriate for this dye. Alexa Fluor™ 488. Blue 488 nm.
Alexa Fluor® 488. Amax 493 Emax 519nm. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC Cy2 and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures
Alexa Fluor ® 488 Phalloidin allows researchers to fluorescently stain the cytoskeleton through the binding of phalloidin to F-actin. This product is intended for use on fixed and permeabilized samples due to the toxicity associated with phalloidin. After reconstitution the stock solution provides enough material to perform 300 assays based on
2 days ago · Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g. a 530/30-nm bandpass
2021-7-23 · Alexa Fluor® 488 CD4 EPR6855 (Alexa Fluor® 488 Anti-CD4 antibody EPR6855 ) "" 2016 IVD POCT
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