2020-8-8 · ClonExpress TM II Exnase TM II Exnase TM II 10 7 cfu/μg 100 cfu/ng Vector
2015-9-15 · The Exnase TM II-based in vitro recombination approach for cloning influenza gene into reverse genetics system Step1 PCR amplified the linear vector and influenza individual fragment using primers listed in Table 1 Step2 the linear vector one influenza PCR amplicon specific for each fragment and Exnase TM II were mixed for in vitro
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2019-3-21 · Ex nase II at37℃for 30 min(F ig.1.). The cloningpro duc tsc anthen be directlytran sfo rmed to com petent cellswith ap ositive rate >95 . ClonExpress MultiS One Step Cloning Kit is anew version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS
2021-1-5 · So we need to use Exnase II (C214 Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA S and S-D614G construct 1. Exanse II 0.5ul recombination Exanse II enzyme 1ul 5X buffer 3.5ul DMT-digested product=total 5 ul
2021-4-5 · According to the manufacturer s instructions of the recombinant enzyme Exnase II the gene fragment was ligated with the vector pET28a by homologous recombination method. The recombinant plasmids transformed into E. coli BL21 (DE3) competent cells. Positive recombinant clones containing the target gene fragment were screened using T7 promoter
2021-3-15 · Exnase Ⅱ 500bpControlInsert(20ng/μl) C112 ClonExpress II One Step Cloning kit.pdf Created Date 6/5/2019 1 12 08 PM
2021-3-15 · Exnase Ⅱ 500bpControlInsert(20ng/μl) C112 ClonExpress II One Step Cloning kit.pdf Created Date 6/5/2019 1 12 08 PM
2018-7-18 · ClonExpress® II One Step Cloning kit is a new version cloning kit based on ClonExpress® One Step Cloning technology supplied with Exnase® II and reaction buffer. Compared to the previous version Exnase® II is added a unique recombinant enhancer which can significantly improve the efficiency of recombination (100 cfu/ ng Vector) even
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2018-7-18 · ClonExpress® II One Step Cloning kit is a new version cloning kit based on ClonExpress® One Step Cloning technology supplied with Exnase® II and reaction buffer. Compared to the previous version Exnase® II is added a unique recombinant enhancer which can significantly improve the efficiency of recombination (100 cfu/ ng Vector) even
2021-3-15 · Exnase Ⅱ 500bpControlInsert(20ng/μl) C112 ClonExpress II One Step Cloning kit.pdf Created Date 6/5/2019 1 12 08 PM
2021-7-20 · ClonExpress ® II Exnase ® II / PCR 5 PCR 5 3 (15 bp-20
The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification greatly simplifying The experimental steps.
2020-8-8 · ClonExpress TM II Exnase TM II Exnase TM II 10 7 cfu/μg 100 cfu/ng Vector
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ClonExpress®II Exnase®II . / PCR 5 PCR 5 3 (15 bp-20 bp) . PCR Exnase®II 30 min
2019-3-21 · Ex nase II at37℃for 30 min(F ig.1.). The cloningpro duc tsc anthen be directlytran sfo rmed to com petent cellswith ap ositive rate >95 . ClonExpress MultiS One Step Cloning Kit is anew version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS
2015-5-27 · Exnase® II 107 cfu/μg 100 cfu/ng Vector Exnase® II PCR
Exnase is Exnase II. I do not recommend accepting the manuscript in its current form. I think there must be a better explanation of why ExnaseII is better than Exnase before the majority of the molecular biologists are willing to accept ExnaseII intra-HR cloning. Below you can find some examples of errors.
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Recombinases. Cre Recombinase ( NEB #M0298) is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites (1). The loxP recognition element is a 34 base pair (bp) sequence comprised of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality (3).
2019-3-21 · Ex nase II at37℃for 30 min(F ig.1.). The cloningpro duc tsc anthen be directlytran sfo rmed to com petent cellswith ap ositive rate >95 . ClonExpress MultiS One Step Cloning Kit is anew version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS
2015-5-27 · Exnase® II 107 cfu/μg 100 cfu/ng Vector Exnase® II PCR
2015-9-15 · The Exnase TM II-based in vitro recombination approach for cloning influenza gene into reverse genetics system Step1 PCR amplified the linear vector and influenza individual fragment using primers listed in Table 1 Step2 the linear vector one influenza PCR amplicon specific for each fragment and Exnase TM II were mixed for in vitro
2017-9-20 · To analyse the subcellular localization of StGAPCs in vivo full-length coding sequences (CDS) of StGAPCs with termination codon was amplified (refer to Table S1 for the primers used) and cloned in to plasmid pH7LIC-N-eGFP with Exnase II (Vazyme China) to construct the vector GFP-StGAPCs for generating the GFP fused target protein in living cells.
2019-1-31 · ClonExpress.pdf ClonExpressTM II One Step Cloning Kit Vazyme Biotech Co. Ltd w w /Web w . v /Tel 400-600-9335 a Vazyme biotech co. ltd. z y /Sales sales m e . c
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2021-3-15 · Exnase Ⅱ 500bpControlInsert(20ng/μl) C112 ClonExpress II One Step Cloning kit.pdf Created Date 6/5/2019 1 12 08 PM
In addition Exnase II is compatible with the reaction systems of restriction enzyme digestion and PCR which means that both the digestion products of vector and the PCR products of insert can be directly used for recombination without purification significantly simplifying the procedures. Product Manual (pdf)
2020-12-3 · (Exnase€ I l) Mutated Vector (Non-Methylated) Transformation Fig 1 A. 43 Exnase© rninRIJÃJ Ill. Ill. Mutation Point A Mutation Point B A Forward Primer Primer B Forward Primer B Reverse Primer Original Vector (Methylated) Methylated Templates Digestion(Dpn l) Linear Amplification (Non-Methy ated) Homologous Recombination (Exnasee Il)
2015-5-27 · Exnase® II 107 cfu/μg 100 cfu/ng Vector Exnase® II PCR
2021-1-5 · So we need to use Exnase II (C214 Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA S and S-D614G construct 1. Exanse II 0.5ul recombination Exanse II enzyme 1ul 5X buffer 3.5ul DMT-digested product=total 5 ul
2021-4-5 · According to the manufacturer s instructions of the recombinant enzyme Exnase II the gene fragment was ligated with the vector pET28a by homologous recombination method. The recombinant plasmids transformed into E. coli BL21 (DE3) competent cells. Positive recombinant clones containing the target gene fragment were screened using T7 promoter
2015-9-21 · Exnase II Up to 20 μl 4 μl 50 400 ng 2 μl 4.5 5 Exnase II5 3
2019-1-31 · ClonExpress.pdf ClonExpressTM II One Step Cloning Kit Vazyme Biotech Co. Ltd w w /Web w . v /Tel 400-600-9335 a Vazyme biotech co. ltd. z y /Sales sales m e . c
0024 Exnase IIPCR pNZ8148 pNZ8148_bshl pNZ8148_bsh2 Lactococcus lactis NZ9000 Lactococcus lactis NZ9000-pNZ8148_bshl
2020-1-6 · The ClonExpress II is a novel cloning Kit independent of DNA ligase significantly reducing the vself-ligated colonies and bringing a true positive rate > 95 . The enhanced Exnase II and highly optimized buffer significantly improve the recombination efficiency and the tolerance to impurities.
2020-12-1 · A CCAAT-box transcription factor SlNFY10 is a candidate regulator of ascorbic acid biosynthesis. GME1 and GME2 are two homologous genes
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